human brain large insert cdna library Search Results


human fetal brain large-insert cdna library  (Becton Dickinson)
90
Becton Dickinson human fetal brain large-insert cdna library
Upregulation of septin 3 isoforms during RA-induced neural differentiation of SH-SY5Y cells. SH-SY5Y cells were treated for 4 days with vehicle (–) or 2.5 μM RA (+). (a) <t>cDNA</t> synthesized from mRNA of treated cells was used as template for PCR. Three sets of primers were used to detect mRNAs for septin <t>3A,</t> <t>3B,</t> and GAPDH as an internal control. The expected sizes of septin 3A (top), 3B (middle), and GAPDH (bottom) mRNAs were 1160, 1308, and 1000 bp, respectively. (b) Lysates from treated cells were separated by 10% SDS-PAGE and then subjected to Western blotting (WB) with antibodies against septin 3A (top), 3B (middle), or β-actin as an internal loading control (bottom).
Human Fetal Brain Large Insert Cdna Library, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+brain+large+insert+cdna+library/pmc05991146-79-19-25?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
human fetal brain large-insert cdna library - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Upregulation of septin 3 isoforms during RA-induced neural differentiation of SH-SY5Y cells. SH-SY5Y cells were treated for 4 days with vehicle (–) or 2.5 μM RA (+). (a) cDNA synthesized from mRNA of treated cells was used as template for PCR. Three sets of primers were used to detect mRNAs for septin 3A, 3B, and GAPDH as an internal control. The expected sizes of septin 3A (top), 3B (middle), and GAPDH (bottom) mRNAs were 1160, 1308, and 1000 bp, respectively. (b) Lysates from treated cells were separated by 10% SDS-PAGE and then subjected to Western blotting (WB) with antibodies against septin 3A (top), 3B (middle), or β-actin as an internal loading control (bottom).

Journal: Gene Expression

Article Title: Expression of Septin 3 Isoforms in Human Brain

doi:

Figure Lengend Snippet: Upregulation of septin 3 isoforms during RA-induced neural differentiation of SH-SY5Y cells. SH-SY5Y cells were treated for 4 days with vehicle (–) or 2.5 μM RA (+). (a) cDNA synthesized from mRNA of treated cells was used as template for PCR. Three sets of primers were used to detect mRNAs for septin 3A, 3B, and GAPDH as an internal control. The expected sizes of septin 3A (top), 3B (middle), and GAPDH (bottom) mRNAs were 1160, 1308, and 1000 bp, respectively. (b) Lysates from treated cells were separated by 10% SDS-PAGE and then subjected to Western blotting (WB) with antibodies against septin 3A (top), 3B (middle), or β-actin as an internal loading control (bottom).

Article Snippet: The human cDNA fragments of septin 3A, 3B, and Nedd5 were produced by polymerase chain reaction (PCR) from the Human Fetal Brain Large-Insert cDNA library (BD Biosciences Clontech, Palo Alto, CA).

Techniques: Synthesized, SDS Page, Western Blot